Meera Jasmine was born in a Syrian Christian family in Thiruvalla, Pathanamthitta district, Kerala, India, to Joseph and Aleyamma. She completed her schooling in Bala Vihar, Thiruvalla and Marthoma Residential School, Thiruvalla. She appeared for her Higher Secondary Exams in March 2000. She had enrolled for a BSc degree in Zoology at Assumption College, Changanassery and completed nearly three months when she was spotted by director Blessy (who was then an assistant director to director Lohithadas) and offered a role in Soothradharan.
Meera Jasmine became noted in the Telugu film industry with Run, the dubbed version of the same-titled Tamil film. She was in the Telugu films in 2004 with Ammayi Bagundi and Gudumba Shankar but also entered Kannada cinema by co-starring with Puneet Rajkumar in Maurya. Her Kannada film Arasu again with Puneet Rajkumar and Ramya is a hit. Her other Kannada films include Devaru Kotta Thangi and Ijjodu.
Meera Jasmine's biggest commercial success in Telugu remains Bhadra with Ravi Teja in the male lead. Her other Telugu films are Raraju, Maharadhi, Yamagola Malli Modalayindi, Gorintaku and Maa Ayana Chanti Pilladu, in which she is paired for a second time with Sivaji.