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FAME has a lower energy content than diesel due to its oxygen content, and as a result, performance and fuel consumption can be affected. It also can have higher levels of NOx emissions, possibly even exceeding the legal limit. FAME also has lower oxidation stability than diesel, and it offers favorable conditions for bacterial growth, so applications which have a low fuel turnover should not use FAME.[33] The loss in power when using pure biodiesel is 5 to 7%.[30]
Fuel equipment manufacturers (FIE) have raised several concerns regarding FAME fuels: free methanol, dissolved and free water, free glycerin, mono and diglycerides, free fatty acids, total solid impurity levels, alkaline metal compounds in solution and oxidation and thermal stability. They have also identified FAME as being the cause of the following problems: corrosion of fuel injection components, low-pressure fuel system blockage, increased dilution and polymerization of engine sump oil, pump seizures due to high fuel viscosity at low temperature, increased injection pressure, elastomeric seal failures and fuel injector spray blockage